Step 1
Using a pencil, write the patient's name and birth date on the frosted end of the microscope slide.

Step 2
Expose the uterine cervix to view and identify the cervical os.

Step 3
Insert the central bristles of the brush into the endocervical canal.  Use gentle pressure in the direction of the cervix until the lateral bristles bend against the ectocervix.

Step 4
Maintaining the gentle pressure, rotate the Cervex-Brush® four to five times in a clockwise direction, by rolling the shaft between thumb and forefinger.

Step 5
Transfer the sample to the microscope slide with a painting action, applying first one side of the bristles and then the other.

Step 6
Immediately apply fixative to the slide.

Special Note:
Maintain approximately 6" between slide and fixative spray bottle.  Spray bottles are usually designed to deliver a very fine mist vs. a jet of fixative.  The latter can result in cells being blown from the slide, resulting in a less than adequate smear.